Mitsuzuri Ayako (美綴 綾子, Mitsuzuri Ayako?) is a character in Fate/stay-night in Year 2 Class A. She is a stellar athlete, and captain of the archery dojo. Ayako and Tohsaka Rin actively compete to see who will be the first to snag a boyfriend. In Fate/stay Night, she is victimized by Rider or Caster (depending on the scenario) early in the story and later found unconscious in an alleyway.
In the anime, Ayako is seen with Shinji after her disappearance, although Shinji denies it when asked by Shirou.
Ayako often asks Shirou to come to the archery dojo and watch them practice.